講演情報
[8p-PA2-10]Label-free Detection of Tongue Carcinoma and Lymph Node Metastasis Using a Scanning Point Terahertz Source Microscope
〇(M2)AO N SHEN1, Masaki Arioka2, Shin Ishikane2, Fumi Takahashi2, Takahiro Teramoto3, Kazunori Serita1,3 (1.Waseda University, 2.University of Occupational and Environmental Health, 3.The Universit of Kitakyushu)
キーワード：
THz System、THz Application、THz generation and detection
Pathological diagnosis of focal lesions in tissues relies largely on histological observation using stained tissue sections [1]. Terahertz (THz, 0.1 – 10 THz) waves are attractive for label-free tissue evaluation because of their sensitivity to tissue hydration and dielectric properties. However, their long wavelength limits spatial resolution (1 THz ≈ 300 μm) and makes evaluation of micrometer-scale lesions difficult [2]. To address this limitation, we have developed a scanning point THz source (SPoTS) microscope [3], as shown in Fig. 1. A point THz source with a diameter of approximately 20 µm is generated at the irradiation spot of a femtosecond pulse laser through optical rectification in a nonlinear optical crystal and scanned across the sample surface placed on the crystal. After detecting transmitted or reflected THz pulses using a typical THz time-domain spectroscopic technique, high-spatial-resolution THz imaging and spectroscopy beyond the diffraction limit can be achieved. In this study, we applied this technique to mouse tongue carcinoma and mouse lymph node tissues for label-free histopathological evaluation.
Figure 2(a) shows a reflected THz image of a 15-µm-thick paraffin-embedded tissue section obtained from a mouse tongue carcinoma, together with the corresponding hematoxylin and eosin (HE)-stained image shown in Fig. 2(b). High-resolution THz imaging of the tongue carcinoma was successfully achieved. The carcinoma and normal tissue regions identified in the THz image were in good agreement with those observed in the corresponding HE-stained image. These results demonstrate the potential of THz imaging to distinguish carcinoma from normal tissue without staining. Detailed results and analyses, including THz imaging of micrometastatic lesions in lymph node tissues, will also be presented.
Figure 2(a) shows a reflected THz image of a 15-µm-thick paraffin-embedded tissue section obtained from a mouse tongue carcinoma, together with the corresponding hematoxylin and eosin (HE)-stained image shown in Fig. 2(b). High-resolution THz imaging of the tongue carcinoma was successfully achieved. The carcinoma and normal tissue regions identified in the THz image were in good agreement with those observed in the corresponding HE-stained image. These results demonstrate the potential of THz imaging to distinguish carcinoma from normal tissue without staining. Detailed results and analyses, including THz imaging of micrometastatic lesions in lymph node tissues, will also be presented.