講演情報
[8p-PA3-36]Selective heavy metal sensing using L-cysteine modified Gold nanoparticles
〇(D)Sharmila Sajankila Nadumane1, Rajib Biswas2, Nirmal Mazumder1 (1.MSLS, MAHE, Manipal, 2.Tezpur Univ)
キーワード：
Gold nanoparticles、Heavy metal、Optical detection
Heavy metal toxicity is a major issue resulting from concentrations of heavy metals exceeding the
permissible amount. Heavy metals such as Lead (Pb) and Mercury (Hg) exhibit toxicity even at
very low concentration. Whereas Iron (Fe), Cobalt (Co), Zinc (Zn) show toxicity only at higher
concentrations. Exposure to these heavy metals affect various organs of the body such as kidney,
lungs, lungs, gastro- intestine etc. Conventional techniques such as Atomic Absorption
spectrometry (AAS), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Raman
Spectrometry can be used in the quantification of these heavy metals. However, despite their
advantages such as high sensitivity and accuracy, these techniques are complex, expensive and
require expert personnel to operate. Colorimetry based detection is considered as one of the simple
techniques that helps in the visual detection of heavy metals. Colorimetry based techniques use
different chemical reagents and nanoparticles as sensors in selectively detecting heavy metals. Gold nanoparticles (AuNP) are used as sensors in detecting various analytes or pollutants. The surface of
the AuNPs can be modified using different functionalizing agents such as amino acids, DNA
zymes, chemical reagents for the specific detection. In this study, we used L-cysteine, an amino
acid as the functionalizing agent for AuNP. L- cysteine with carboxyl, amino group and thiol group
will form a stable bond with AuNPs and can be used for the selective detection of Lead and
Mercury. The UV- vis spectra of solution showed a peak at 520 nm specific to AuNPs. In the
presence of Pb and Hg, aggregation was observed causing the solution’s color shift from red to blue
and resulting in additional spectra at 675 nm for Pb and 725 nm for Hg. The correlation coefficient
of Pb and Hg was found to be 0.894 and 0.861 respectively.
permissible amount. Heavy metals such as Lead (Pb) and Mercury (Hg) exhibit toxicity even at
very low concentration. Whereas Iron (Fe), Cobalt (Co), Zinc (Zn) show toxicity only at higher
concentrations. Exposure to these heavy metals affect various organs of the body such as kidney,
lungs, lungs, gastro- intestine etc. Conventional techniques such as Atomic Absorption
spectrometry (AAS), Inductively Coupled Plasma Mass Spectrometry (ICP-MS), Raman
Spectrometry can be used in the quantification of these heavy metals. However, despite their
advantages such as high sensitivity and accuracy, these techniques are complex, expensive and
require expert personnel to operate. Colorimetry based detection is considered as one of the simple
techniques that helps in the visual detection of heavy metals. Colorimetry based techniques use
different chemical reagents and nanoparticles as sensors in selectively detecting heavy metals. Gold nanoparticles (AuNP) are used as sensors in detecting various analytes or pollutants. The surface of
the AuNPs can be modified using different functionalizing agents such as amino acids, DNA
zymes, chemical reagents for the specific detection. In this study, we used L-cysteine, an amino
acid as the functionalizing agent for AuNP. L- cysteine with carboxyl, amino group and thiol group
will form a stable bond with AuNPs and can be used for the selective detection of Lead and
Mercury. The UV- vis spectra of solution showed a peak at 520 nm specific to AuNPs. In the
presence of Pb and Hg, aggregation was observed causing the solution’s color shift from red to blue
and resulting in additional spectra at 675 nm for Pb and 725 nm for Hg. The correlation coefficient
of Pb and Hg was found to be 0.894 and 0.861 respectively.