講演情報
[9p-PB7-5]Tip-Enhanced Raman Spectroscopy of lipid bilayer in non-gap-mode
〇(M2)Nao Kuroki1, Takayuki Umakoshi1, Prabhat Verma1 (1.UOsaka)
キーワード：
Tip-enhanced Raman spectroscopy、Nanoscale chemical imaging、Lipid bilayer
The cell membrane is a platform for many cellular processes, and its function is related to the nanoscale distribution of biomolecules such as lipids and proteins. Therefore, nanoscale chemical analysis is required to understand membrane properties. Tip-enhanced Raman spectroscopy (TERS) enables Raman spectroscopy with nanoscale spatial resolution by using near-field light localized at a metallic tip. Although lipid bilayers have been investigated by gap-mode TERS, this method requires a metal substrate and is not suitable for thicker samples such as cells. In this study, we demonstrate non-gap-mode TERS detection of lipid bilayers as a model of the cell membrane. A silver-coated AFM tip with a rough plasmonic surface was prepared to enhance the near-field light, and tapping-mode AFM was employed to reduce damage to the soft lipid bilayer. Characteristic lipid Raman peaks in the C–H stretching region were detected only when the metallic tip approached the sample, confirming tip-induced signal enhancement. These results demonstrate the potential of non-gap-mode TERS for nanoscale chemical imaging of lipid bilayers and future cell membrane analysis.