講演情報
[V-16-27]Recombinant mouse interleukin 36 γ secreted by Lactococcus lactis induces Mucin2 mRNA expression in mouse colon cells
*髙瀨 渉吾1、生井 楓1、重盛 駿1、下里 剛士1 (1. 信州大バイオメディカル研)
Microbial therapeutics have been researched for use in livestock animals. The intestinal mucus barrier prevents bacterial infections and maintains host-microbiota homeostasis. Recently, it has been reported that interleukin (IL) -36γ serves as a signal that induces mucin expression. We hypothesized that the delivery of IL-36γ to intestinal mucosa improves the gut environment. In this study, we constructed a genetically modified lactic acid bacteria (gmLAB) that secretes IL-36γ. The mouse (m) IL-36γ gene was inserted into the lactococcal secretion vector pNZ8148#2:SEC, and the resulting plasmid was introduced into Lactococcus lactis to generate a gmLAB designated NZ-IL36γ. The band corresponding to recombinant mIL-36γ was detected (by western blot) in the supernatant of NZ-IL36γ cultured with nisin. Next, we investigated whether mIL-36γ produced by NZ-IL36γ exhibits bioactivity in intestinal epithelial cells of a mouse colon cell line, CMT93/69; mIL-36γ purified from the gmLAB supernatant was compared to commercially available mIL-36γ. As a result, The addition of purified mIL-36γresulted accumulation of the Mucin 2.