講演情報
[I-P03-5-05]急性心筋炎の原因ウイルス同定におけるターゲットエンリッチメントメタゲノミクスの応用
○中釜 瞬1, 中釜 悠1, 篠原 美香1, 中釜 幸恵1, 田中 優2, 安田 和志2, 元岡 大祐3, 植田 智美4, 伊藤 正道4, 網谷 英介4, 犬塚 亮4 (1.大阪公立大学 医学研究科, 2.あいち小児保健医療総合センター, 3.大阪大学 微生物学研究所, 4.東京大学医学部附属病院)
キーワード：
acute myocarditis、metagenomics、parvovirus B19
Introduction and Objective: Our attempts to delineate the causative pathogens of human myocarditis, including the early applications of the VirCapSeq-VERT platform target-enrichment metagenomics, have not yet led to definitive findings. A preliminary report demonstrated the simultaneous detection of another co-infecting cardiotropic virus in 20% of parvovirus B19 (PVB19)-positive endomyocardial biopsy (EMB) specimens. The study aimed to redefine the viral etiological landscape in myocarditis cases through a virome-wide target-enrichment metagenomics approach. Methods: EMB specimen-derived DNA/RNA were obtained from consecutive myocarditis cases. An integrated molecular approach combining PCR-based methods and target-enrichment metagenomics (Twist Comprehensive Viral Research Panel) was employed to explore the viral etiology within them. Blood-derived DNA/RNA from a healthy donor served as our negative control. Results: 4/10 of the EMB specimen were positive for PVB19. Sequence reads from metagenomics showed co-detection of other cardiotropic viruses in all PVB19-positive cases. PCR-based confirmatory detection of these co-detected cardiotropic viruses is either still pending or has so far been unsuccessful.Discussion and Conclusion: By applying target-enrichment metagenomics, we co-detected the likely causative cardiotropic viruses from EMB specimen. Although the study design is limited by the lack of an effective control case, our observations suggest that viral interplay, which may alter pathogens’ behaviors, may contribute to the pathology of myocarditis.