講演情報
[II-TKIS-OR-3]Dynamics of SOX17-expressing endothelial precursors during vascular network formation integrating extraembryonic and embryonic circulation
○Rie Saba1, Naoki Takeshita1,2, So Miyanishi1, Kana Kitazaki1, Kenta Yashiro1 (1.Department of Anatomy, Kyoto Prefectural University of Medicine, Kyoto, Japan, 2.Department of Pediatrics, Kyoto Prefectural University of Medicine, Kyoto, Japan.)
キーワード：
SOX17、endothelial network、3D imaging
The establishment of a vascular endothelial network spanning extraembryonic and embryonic tissues is a critical morphogenetic process that supports subsequent organogenesis by integrating embryonic and extraembryonic circulation. However, the cellular dynamics and molecular mechanisms that coordinate the formation of this network remain incompletely understood.
SOX17 plays multiple pivotal roles during cardiogenesis and vasculogenesis. As we reported previously, Sox17 is expressed in endocardium and endothelial precursors, and loss of mesodermal Sox17 results in not only impaired vascular remodeling in both extraembryonic and embryonic tissues but also heart malformations. These findings suggest that analysis of spatiotemporal dynamics of SOX17-expressing (+) cells can offer insights into the mechanisms of the endothelium network formation connecting extraembryonic and embryonic tissues. Nevertheless, the precise distribution and dynamic behavior of SOX17(+) precursors remain unclear.
To address these, we combined immunohistochemistry and three-dimensional imaging of the mouse embryo within the yolk sac. Using genetic reporter and lineage-tracing approaches, we visualized vascular endothelial network formation and examined the spatiotemporal dynamics of mesodermal SOX17(+) cells. Our results demonstrate that mesodermal SOX17(+) cells are a highly primitive progenitor population contributing to the cardiovascular endothelial network that connects embryonic and extraembryonic circulation.
These findings advance our understanding of early vascular integration.
SOX17 plays multiple pivotal roles during cardiogenesis and vasculogenesis. As we reported previously, Sox17 is expressed in endocardium and endothelial precursors, and loss of mesodermal Sox17 results in not only impaired vascular remodeling in both extraembryonic and embryonic tissues but also heart malformations. These findings suggest that analysis of spatiotemporal dynamics of SOX17-expressing (+) cells can offer insights into the mechanisms of the endothelium network formation connecting extraembryonic and embryonic tissues. Nevertheless, the precise distribution and dynamic behavior of SOX17(+) precursors remain unclear.
To address these, we combined immunohistochemistry and three-dimensional imaging of the mouse embryo within the yolk sac. Using genetic reporter and lineage-tracing approaches, we visualized vascular endothelial network formation and examined the spatiotemporal dynamics of mesodermal SOX17(+) cells. Our results demonstrate that mesodermal SOX17(+) cells are a highly primitive progenitor population contributing to the cardiovascular endothelial network that connects embryonic and extraembryonic circulation.
These findings advance our understanding of early vascular integration.