講演情報
[BP-5]古代人由来全ゲノムシーケンシングデータを用いた古代バイローム解析
○西村 瑠佳1,2, 杉本 竜太2, 井ノ上 逸朗1,2 (1.総合研究大学院大学 生命科学研究科 遺伝学専攻, 2.国立遺伝学研究所 人類遺伝研究室)
※12月15日（木）と16日（金）で同一演題の発表を行います。
Ancient DNA can be extracted from ancient people’s bones and mummified tissues. These DNA contains genomic information of ancient bacteria and viruses that infected ancient people. Since 1997, ancient viral genomes such as influenza A virus and hepatitis B virus have revealed the past pandemic and the long-term viral evolution. Most of the detected ancient viruses are pathogenic ones, and the number of ancient viruses is limited. Here, we examined the public whole genomic sequencing (WGS) data derived from archaeological samples such as bones, coprolites, and dental calculus. More than ten public WGS data were downloaded and analyzed based on alignment and assembly. We obtained several non-pathogenic viral sequences such as Streptococcus phage. Non-homologous viral search using CRISPR immunological memories of host bacteria revealed more than 50 novel viral sequences in ancient samples, which cannot be seen in the current database. We then compared the viral components of each ancient sample to characterize ancient virome. Our results suggested that the WGS data of archaeological samples might be helpful to the ancient viral genomes and analyzing ancient viromes.