講演情報
[O33-1]広汎子宮頸部摘出術後妊娠における妊娠初期腟分泌物細菌叢解析
○春日 義史1, 大西 英理子2, 嘉村 浩美2, 長谷川 慶太1,2, 福間 優花1, 葉室 明香1, 玉井 順子1, 田中 雄也1, 池ノ上 学1, 中林 一彦1, 田中 守1, 秦 健一郎2 (1.慶應義塾大学 医学部 産婦人科, 2.国立成育医療研究センター 周産期病態研究部)
Objective: Pregnancy after radical trachelectomy (RT) predisposes to a higher risk of preterm delivery. Since there is a lack of data on vaginal microbiota in pregnancy after RT, we investigated the vaginal microbiota at the first trimester.Methods: Pregnancies after RT that delivered after 22 gestational weeks between June 2019 and December 2021 at our hospital were included. Medical doctors collected vaginal discharge samples between 10 and 14 gestational weeks. Vaginal microbiota were analyzed by sequencing the V1-V2 region of the 16S ribosomal RNA gene. We compared the frequency of non-Lactobacillus-dominated vaginal microbiota, defined as ＜ 98% Lactobacillus spp., between the RT group (n = 12) and non-RT group (n = 72).Results: Seven out of the 12 participants delivered preterm due to preterm premature rupture of membrane (n = 2, 29%), labour onset (n = 4, 57%), and pulmonary thromboembolism (PTE) (n = 1, 14%). The frequency of non-Lactobacillus-dominated vaginal microbiota was not significantly different between the RT and non-RT groups (50% vs 40%, p = 0.54). In the RT group, the frequency of non-Lactobacillus-dominated vaginal microbiota was not different between full-term delivery and preterm delivery, excluding the PTE case (40% vs 67%, p = 0.58).Conclusion: The frequency of non-Lactobacillus-dominated vaginal microbiota at the first trimester appeared to be higher in the RT group than in the non-RT group. Furthermore, the frequency of non-Lactobacillus-dominated vaginal microbiota in preterm delivery was higher than that in full-term delivery in the RT group.