講演情報

[OE5-4]日本人787名の腸内微生物叢データからの原核生物及びウイルスゲノムの再構築及びその特徴の解明

^○友藤嘉彦¹, 岸川敏博 ¹, 前田悠一 ¹, 小河浩太郎 ¹, 大竹由利子 ¹, 川端修平 ¹, 新居卓郎 ¹, 奥野龍禎 ¹, 猪頭英里 ¹, 木下允 ¹, 高垣匡寿 ¹, 大山直紀 ³, 藤堂謙一 ¹, 山本賢一 ¹, 曽根原究人 ¹, 八木田麻裕 ¹, 細川明子 ⁴, 元岡大祐 ², 松本悠希 ², 松岡秀俊 ⁷, 吉村麻衣子 ⁷, 大島至郎 ⁷, 新﨑信一郎 ¹, 中村昇太 ², 飯島英樹 ¹, 猪原秀典 ¹, 貴島晴彦 ¹, 竹原徹郎 ¹, 望月秀樹 ¹, 竹田潔 ¹, 熊ノ郷淳 ¹, 岡田随象 ^1,5,6 (1.大阪大学大学院医学系研究科, 2 大阪大学微生物病研究所, 3 川崎医科大学, 4 吹田市民病院, 5 東京大学大学院医学系研究科, 6 理化学研究所生命医科学研究センター, 7 国立病院機構大阪南医療センター)

Microbial genomes recovered from the gut metagenome sequencing reads are important resources for studying the gut microbiome. However, the current populational diversity of the prokaryotic genomes is still limited because the number of microbial genomes recovered from populations other than European, North American, and Chinese is relatively low.Here, we reconstructed 19,084 prokaryotic and 31,395 viral genomes from the 787 Japanese gut metagenome shotgun sequencing data as Japanese Metagenome Assembled Genomes (JMAG) and Japanese Virus Database (JVD) and made them publicly available.Population-specific enrichment of the Bacillus subtilis and β-porphyranase among the JMAG could derive from the Japanese traditional food natto and nori, respectively. Five food-associated species (four dairy-related species and natto-related Bacillus subtilis) were shared among the Japanese at the strain level and two dairy-related species were nominally associated with the East Asian-specific missense variant rs671 in ALDH2 which was associated with dairy consumption. As for the viral genomes, 62.9% of the species-level clusters in the JVD were novel. The composition of the β crAss-like phages was low among Japanese but relatively high among the Africans and Oceanians. Several clades of the crAss-like phages decreased in rheumatic diseases and inflammatory bowel diseases but increased in colorectal cancer. Analysis on the CRISPR sequences significantly expanded the catalog of the virus-host pairs and revealed that abundances of the viruses and their hosts tended to be positively correlated.

セッション詳細へ戻る