講演情報
[OE9-5]長鎖シークエンス技術を用いた成人T細胞白血病のトランスクリプトーム解析
○藤本 明洋1, 田中 梓1, 栗林 嶺至1, 安永 純一郎2, 松岡 雅雄2 (1.東京大学 医学系研究科, 2.熊本大学 医学部 血液内科)
Adult T-cell leukemia-lymphoma (ATL) is a poor prognostic T-cell lymphoma caused by infection of T-cell leukemia virus type 1 (HTLV-1). To investigate the mechanism of leukemogenesis, genome, transcriptome, and epigenetic studies have been performed. Although driver genes and epigenetic abbreviations were identified in previous studies, our understanding of the molecular mechanism of ATL is still far from complete. To analyze the transcriptional abnormalities, we analyzed full-length transcripts for ATL using a long-read sequencer (Oxford Nanopore). We sequenced cDNA from RNA from 26 ATL samples and eight controls from CD4+ T-cell, and obtained approximately 19.8M reads/sample. cDNA sequence data were analyzed by SPLICE software (Kiyose et al. (under review)). A total of 55,308 and 37,938 non-redundant transcripts were identified in ATLs and controls, of which 8-10% were novel. A comparison of the expression level between ATL and CD4+ T-cell identified 7046 significantly differentiated transcripts (DETs) in 4025 genes. Interestingly, 1553 genes with DETs were not found by a gene-level analysis. Furthermore, fusion-gene detection showed novel recurrent fusion events that were not detected by previous studies. These results suggest the efficiency of full-length transcriptome studies and the importance of splicing variants in the leukemogenesis of ATL.