Presentation Information

[3F01]Evaluation of the Fast-Neutron Flux of the Irradiation Field in KUANS for Fast-Neutron-Based Plant Breeding

*Mizuki Iriyama1, Seiji Tasaki1, Masahiro Hino1, Shuhei Nasuda1, Kanata Senoo1 (1. Kyoto Univ.)

Keywords:

plant breeding,fast neutron,compact neutron source

Fast neutrons have strong penetration and can deposit high energy uniformly throughout plant seed, offering a complementary mutation-breeding method to conventional techniques. In this study, we use the region near the neutron-production target of the Kyoto University Accelerator-driven Neutron Source (KUANS), as a fast-neutron irradiation field and, in collaboration with the Breeding Science Laboratory of the Graduate School of Agriculture, have established an integrated framework covering irradiation, cultivation, and analysis. Our purpose is to quantify the scale and distribution of fast-neutron-induced mutations through genome analysis of the stabilized M3 generation. To achieve this goal, we irradiated wheat and Arabidopsis seed at different dose levels. We also developed an irradiation cell with a graphite reflector and conducted fast-neutron flux evaluations essential for assessing mutation-induction effects. This presentation reports on these irradiation experiments and flux-evaluation results.