The 16th Symposium on Biorelevant Chemistry
Sep 10 - Sep 12, 2022Held On-site and Online
The 16th Symposium on Biorelevant Chemistry
Sep 10 - Sep 12, 2022Held On-site and Online
[1B-01]Sesamin oxidation mechanism of CYP92B14 related to lignan accumulation in seseme seeds
*Erisa Harada1, Jun Murata1, Kei Shiraishi1, Hiromi Toyonaga1, Eiichiro Ono2, Masayuki P. Yamamoto3, Manabu Horikawa1(1. Suntory foundation of Life sciences, 2. Research Institute, Suntory Global Innovation Center Ltd, 3. Graduate School of Science and Engineering, University of Toyama)
Keywords:
cyochrome P450,sesamin oxidizing enzyme,lignan
ゴマ栽培種のセサミン酸化酵素CYP92B14は、芳香環の直接酸化と、oxidative rearrangement of a-oxy-substituted aryl groups の２種類の酸化反応で、セサミノールとセサモリンを生成する。野生種ホモログ酵素の同定、および変異体解析から、新たな酸化反応経路の特定と生成物の生成比の逆転に成功し、生成物比を決めるアミノ酸残基を明らかにした。