Presentation Information
[3SBT-14-KL]High-Temperature Ethanol Fermentation: Linking Yeast Stress Biology to Industrial Application
○Choowong Auesukaree1,2 (1. Department of Biotechnology, Faculty of Science, Mahidol University (Thailand), 2. Mahidol University-The University of Osaka Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University (Thailand))
Keywords:
Bioethanol,Cellular Response,High-Temperature Fermentation,Stress Tolerance,Yeast
High-temperature ethanol fermentation represents a promising strategy for improving the energy efficiency of bioethanol production, particularly for large-scale industrial processes in tropical regions where ambient temperatures frequently exceed 35 °C. Fermentation at elevated temperatures offers several advantages, including reduced cooling costs, minimized risk of bacterial contamination, and improved compatibility with simultaneous saccharification and fermentation. However, under these conditions, yeast cells are simultaneously exposed to multiple stresses, including heat, ethanol toxicity, high osmolarity, and oxidative stress, which collectively limit fermentation performance and process stability.
This presentation highlights recent advances in yeast stress biology that are critical for enhancing efficient high-temperature ethanol fermentation. Naturally thermotolerant Saccharomyces cerevisiae strains capable of efficient ethanol production above 40 °C, identified and characterized by our group, were used as model systems for multiple stress tolerance. To elucidate cellular responses to fermentation-associated stresses, a combined approach integrating conventional molecular genetic techniques with multi-omics analyses was employed. The results indicate that coordinated regulation of heat shock responses, redox homeostasis, mitochondrial function, and cell wall and membrane lipid remodeling plays a central role in conferring tolerance to high-temperature fermentation-associated stresses and in maintaining cellular homeostasis during prolonged fermentation.
In addition to these biological insights, strategies for translating yeast stress tolerance into improved industrial performance, as well as key challenges toward industrial deployment, will be discussed. Altogether, this work illustrates how mechanistic insights into yeast stress biology can be leveraged to advance high-temperature ethanol fermentation as a viable biotechnology for sustainable biofuel production.
This presentation highlights recent advances in yeast stress biology that are critical for enhancing efficient high-temperature ethanol fermentation. Naturally thermotolerant Saccharomyces cerevisiae strains capable of efficient ethanol production above 40 °C, identified and characterized by our group, were used as model systems for multiple stress tolerance. To elucidate cellular responses to fermentation-associated stresses, a combined approach integrating conventional molecular genetic techniques with multi-omics analyses was employed. The results indicate that coordinated regulation of heat shock responses, redox homeostasis, mitochondrial function, and cell wall and membrane lipid remodeling plays a central role in conferring tolerance to high-temperature fermentation-associated stresses and in maintaining cellular homeostasis during prolonged fermentation.
In addition to these biological insights, strategies for translating yeast stress tolerance into improved industrial performance, as well as key challenges toward industrial deployment, will be discussed. Altogether, this work illustrates how mechanistic insights into yeast stress biology can be leveraged to advance high-temperature ethanol fermentation as a viable biotechnology for sustainable biofuel production.
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