[P003] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P02-191] Engineering multi-layered biocomputing circuits and metabolic coupling for controlled and stable bioproduction

○Bingyin Peng¹ (1. The University of Queensland (Australia))

Keywords:

Genetic circuit,Metabolic engineering,Terpenoids,Yeast,Metabolic coupling

Microbial cells exhibit genetic plasticity and are capable of rapidly adapting to new environmental niches to enhance their growth fitness, either through inherited mechanisms of genetic reprogramming or spontaneous mutagenesis. This hinders the long-term stability of both synthetic regulatory and metabolic systems and causes strain degeneration. We aim to develop computer-like synthetic circuit systems to programme microbial metabolism and proliferation in controlled sequences, tailored for optimisation in strain development and stabilised industrial production. Here, we use the common budding yeast Saccharomyces cerevisiae as the chassis for hyperactive production of heterogeneous terpenoid products. We engineered multi-layered regulatory circuit systems for staged control over yeast growth and heterologous production. We also developed a multi-colour biosensing/reporting system to understand the genetic responses and the population stability in yeasts engineered with stressful or imbalanced terpene-synthetic pathways. Furthermore, we prototyped a metabolic coupling system that allows long-term evolutionary stability for heterologous production and genetic circuit systems.