Presentation Information
[P02-207]Phyto-Engineered Silver Nanoparticles from Mikania micrantha: A Multifunctional Platform for Biocatalysis and Advanced Therapeutics
○Manthae C Phom1, Tovishe Phucho1 (1. Nagaland University (India))
Keywords:
Biogenic AgNPs,,Biocatalysis,Multifunctional nanomaterials,Anticancer activity
[Purpose]
Silver nanoparticles (AgNPs) synthesized through eco-friendly methods are gaining significant attention for their diverse applications. In this study, we successfully biosynthesized AgNPs using the aqueous leaf extract of Mikania micrantha, which naturally provides the phytochemicals needed to reduce and stabilize the nanoparticles.
[Method]
By optimizing the process, we found the ideal conditions to be a 5% (v/v) extract dosage, pH 9, and 80°C for 60 minutes. The synthesized nanoparticles were evaluated through thorough characterization employing UV–Vis, FT-IR, XRD, FE-SEM, EDX, TEM, and SAED.
[Results]
Characterization revealed highly crystalline, spherical nanoparticles with a face-centered cubic structure, ranging from 12.35 to 46.83 nm in size. They drove the acid-free Biginelli reaction to excellent yields in just 30 seconds and facilitated the synthesis of various 2,4,5-triphenyl-1H-imidazole compounds within 30–180 seconds. In biological assays, they achieved 89.8 % radical scavenging activity in the DPPH assay (IC50 = 24.99 ± 0.19 μg/mL) and showed the highest antibacterial inhibition against Yersinia pestis (21 ± 0.3 mm). Furthermore, the AgNPs significantly inhibited human colon carcinoma (Caco-2) cells with an IC50 of 36.17 μg/mL.
[Consideration]
hese biogenic AgNPs proved to be remarkably efficient, eco-friendly catalysts. Beyond their chemical utility, the nanoparticles exhibited potent biological activity. They demonstrated a stronger reducing power than the positive control in the FRAP assay, exhibited broad-spectrum antibacterial efficacy, and showed promising targeted anticancer potential, proving twice as effective as the raw plant extract.
[Conclusion]
Ultimately, this study highlights a sustainable, highly efficient pathway for creating multifunctional nanomaterials.
Silver nanoparticles (AgNPs) synthesized through eco-friendly methods are gaining significant attention for their diverse applications. In this study, we successfully biosynthesized AgNPs using the aqueous leaf extract of Mikania micrantha, which naturally provides the phytochemicals needed to reduce and stabilize the nanoparticles.
[Method]
By optimizing the process, we found the ideal conditions to be a 5% (v/v) extract dosage, pH 9, and 80°C for 60 minutes. The synthesized nanoparticles were evaluated through thorough characterization employing UV–Vis, FT-IR, XRD, FE-SEM, EDX, TEM, and SAED.
[Results]
Characterization revealed highly crystalline, spherical nanoparticles with a face-centered cubic structure, ranging from 12.35 to 46.83 nm in size. They drove the acid-free Biginelli reaction to excellent yields in just 30 seconds and facilitated the synthesis of various 2,4,5-triphenyl-1H-imidazole compounds within 30–180 seconds. In biological assays, they achieved 89.8 % radical scavenging activity in the DPPH assay (IC50 = 24.99 ± 0.19 μg/mL) and showed the highest antibacterial inhibition against Yersinia pestis (21 ± 0.3 mm). Furthermore, the AgNPs significantly inhibited human colon carcinoma (Caco-2) cells with an IC50 of 36.17 μg/mL.
[Consideration]
hese biogenic AgNPs proved to be remarkably efficient, eco-friendly catalysts. Beyond their chemical utility, the nanoparticles exhibited potent biological activity. They demonstrated a stronger reducing power than the positive control in the FRAP assay, exhibited broad-spectrum antibacterial efficacy, and showed promising targeted anticancer potential, proving twice as effective as the raw plant extract.
[Conclusion]
Ultimately, this study highlights a sustainable, highly efficient pathway for creating multifunctional nanomaterials.
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