[P003] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P02-219] Gallic acid modulates copper and iron homeostasis and antioxidant systems in Saccharomyces cerevisiae

○Kittikhun Kerdsomboon^1,2, Todsapol Techo³, Tossapol Limcharoensuk², Choowong Auesukaree^2,4 (1. Chulabhorn International College of Medicine, Thammasat University, Pathum Thani 12120, Thailand (Thailand), 2. Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand (Thailand), 3. Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand (Thailand), 4. Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand (Thailand))

Keywords:

Gallic acid,Saccharomyces cerevisiae,Transcriptomic analysis,Metal homeostasis,Antioxidant system

Gallic acid (GA) is a plant-derived phenolic compound known for its antioxidant and metal-chelating properties; however, the cellular responses to GA remain poorly understood. Here, we investigated the molecular and physiological responses of Saccharomyces cerevisiae BY4742 to GA, with a focus on transcriptomic regulation, metal homeostasis, and antioxidant defense mechanisms. RNA sequencing identified 139 differentially expressed genes, enriched in pathways related to transmembrane transport, secondary metabolism, and amino acid metabolism, whereas steroid biosynthesis was downregulated. GA treatment significantly altered intracellular metal levels, but not phosphate levels. These changes were associated with differential expression of key transporter genes, including upregulation of CTR1 and downregulation of FET3, FTR1, and PHO84. Although high concentrations of GA increased intracellular reactive oxygen species, GA enhanced antioxidant defenses by elevating superoxide dismutase and catalase activities, increasing reduced glutathione levels, and improving the GSH:GSSG ratio. Collectively, these findings demonstrate that GA induces a hormetic response in yeast, promoting adaptive transcriptional reprogramming, modulation of metal homeostasis, and reinforcement of antioxidant systems. This study provides mechanistic insights into the protective effects of GA and supports its potential applications in biotechnology and environmental research.