[P003] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P02-249] Metabolic Engineering of Synechococcus elongatus PCC 7942 for Production of 3-Hydroxypropionate and Its Derivatives

○Lily S. Li¹, Arvin Y. Chen¹, Ethan I. Lan¹ (1. National Yang Ming Chiao Tung University (Taiwan))

Keywords:

cyanobacteria,bioproduction

In this research, we have successfully leveraged the innate photosynthetic efficiency of cyanobacteria to facilitate carbon fixation, producing 3-hydroxypropionic acid (3-HP) at a concentration of 600 mg/L in Synechococcus elongatus PCC 7942. Unlike traditional heterologous hosts, cyanobacteria offer a sustainable 'chassis' that utilizes solar energy and atmospheric CO₂ as the sole carbon source, thereby bypassing the need for expensive sugar feedstocks and avoiding competition with food crops. Building on this robust 3-HP production platform, we expanded the metabolic network toward high-value downstream derivatives. Through the heterologous expression of targeted enzymes, we successfully demonstrated the conversion of 3-HP into propionate and 1-propanol. Preliminary results indicate promising metabolic flux toward these products. Notably, 1-propanol serves as a critical bio-based precursor for polypropylene production and possesses superior energy density and lower hygroscopicity compared to bioethanol, making it a premier candidate for advanced biofuels and green chemical feedstocks. This work demonstrates a high-efficiency photosynthetic 'bio-foundry' for the sustainable synthesis of chemicals directly from CO₂, highlighting the potential of engineered cyanobacteria to decarbonize industrial chemical manufacturing.