[P003] Poster

12:10 PM - 1:00 PM JST(3:10 AM - 4:00 AM UTC)

[P02-267] Optimization of cultivation parameters for enhanced bacterial cellulose production by Komagataeibacter xylinus

○José Manuel Domínguez¹, Mónica Salvador-Cardona¹, Martín Cid-Fernández¹, Aida Ochogavias¹, Ricardo Pinheiro de Souza Oliveira², Alicia Paz¹ (1. Universidade de Vigo (Spain), 2. USP (Brazil))

Keywords:

Bacterial cellulose,Komagataeibacter xylinus,culture optimization,bioprocess conditions,static cultivation,glucose concentration

PurposeBacterial cellulose (BC) is a high-value biopolymer with applications in food, biomedical and advanced materials. However, its production efficiency strongly depends on cultivation conditions. This study aimed to evaluate preliminary culture parameters affecting BC production by Komagataeibacter xylinus, focusing on the effect of initial glucose concentration and cultivation mode.MethodK. xylinus CECT 473 was cultivated in GY medium using two initial glucose concentrations (20 and 50 g/L). The influence of cultivation mode was also evaluated by comparing static and dynamic cultures. During fermentation, glucose consumption and pH evolution were monitored, and BC production was determined after recovery and purification of the cellulose.ResultsBoth glucose concentrations showed similar consumption rates, although a higher percentage of substrate utilization was observed with 20 g/L. Regarding cultivation mode, static cultures produced a homogeneous cellulose pellicle that facilitated recovery and purification, while dynamic conditions generated irregular cellulose aggregates. No significant differences were observed in total BC mass between both cultivation modes.ConsiderationThe results suggest that reducing glucose concentration improves substrate utilization without compromising BC production. Furthermore, static cultivation provides structural advantages that simplify downstream processing of the produced cellulose.ConclusionA glucose concentration of 20 g/L combined with static cultivation conditions represents suitable preliminary parameters for BC production by K. xylinus. These findings contribute to the optimization of culture strategies for bacterial cellulose bioprocesses.