[1B15-1B17] International sess.(3)

3:45 PM - 4:00 PM JST(6:45 AM - 7:00 AM UTC)English

[1B16] Sequential one-pot enzymatic synthesis of lacto-N-tetraose and its multi-sialylated derivatives

○CAIXIA PEI¹, YUGUANG DU², KENJI KAMIYA¹, EIKA W. QIAN¹ (1. Tokyo University of Agriculture and Technology , 2. Institute of Process Engineering, Chinese Academy of Sciences)

Keywords:

One-pot multienzyme,Lacto-N-tetraose,Glycosyltransferases

Lacto-N-tetraose and its sialyl derivatives including sialyllacto-N-tetraose a, di-sialyl, and tri-sialyl sialylated derivatives were enzymatically synthesized from readily available lactoside, and the corresponding monosaccharides using a highly efficient sequential one-pot multienzyme (OPME) strategy. The OPME strategy which combines bacterial glycosyltransferases and sugar nucleotide generation enzymes provides easy access to the biologically important complex oligosaccharides at preparative scale. Also, our work identified α2,6-sialyltransferase show a great potential solution to overcome the challenges in obtaining disialyllacto-N-tetraose (DSLNT) in large amounts for necrotizing enterocolitis (NEC) disease studies and potential therapies.