Presentation Information
[1F16]Biocatalytic conversion of methane to methanol via enzyme-mediated oxidation
○Toshiaki Kamachi1, Hidehiro Ito1, Yuta Noto1 (1. Institute of Science Tokyo)
Keywords:
Methane/Methanol conversion,methane monooxygenase,Enzymatic conversion
In this presentation, I will introduce two approaches to methane-to-methanol conversion using enzymes derived from methanotrophic bacteria, which metabolize methane and grow under mild conditions such as ambient temperature and pressure.
Using genetic engineering techniques, we constructed a methanotrophic bacterium in which the expression of methanol-metabolizing enzymes can be regulated. This enabled us to switch between methanol production and cell growth using the same organism.
Additionally, we present a low-energy methanol production system that harnesses inexhaustible light energy by combining methane monooxygenase—the enzyme responsible for converting methane to methanol—with photosystem II.
Using genetic engineering techniques, we constructed a methanotrophic bacterium in which the expression of methanol-metabolizing enzymes can be regulated. This enabled us to switch between methanol production and cell growth using the same organism.
Additionally, we present a low-energy methanol production system that harnesses inexhaustible light energy by combining methane monooxygenase—the enzyme responsible for converting methane to methanol—with photosystem II.
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