[20a-A305-1~9] 3.3 Biomedical optics (formerly 3.4)

9:00 AM - 9:15 AM JST(12:00 AM - 12:15 AM UTC)English Presentation

[20a-A305-1] Quantitative intra-tissue activity imaging technique for evaluating in vitro cell culture samples by optical coherence tomography

〇(M2)Rion Morishita¹, Toshio Suzuki^2,3, Pradipta Mukherjee¹, Ibrahim Abd El-Sadek^1,4, Tanatchaya Seesan⁵, Yiheng Lim¹, Shuichi Makita¹, Yuki Yamamoto³, Tetsuharu Nagamoto³, Yoshiaki Yasuno¹ (1.COG, Univ. of Tsukuba, 2.Univ. of Tsukuba, 3.HiLung Inc., 4.Damietta Univ., 5.KMITL)

Keywords:

Optical coherence tomography,in vitro organoid,tissue activity imaging

We have developed a quantitative imaging algorithm called variable time window logarithmic intensity variance (VLIV) to assess in vitro cell culture samples. VLIV computes two quantitative metrics known as damping factor (DF) and magnitude factor (MF). These metrics provide label-free visualization of the speed and magnitude of intra-tissue and intra-cellular activities. We have applied this method to human induced pluripotent stem cell-derived alveolar organoids and their fibrotic models. DF and MF images revealed the morphological and functional structures within the alveolar organoids, such as alveoli and fibroblasts. Additionally, abnormal activity distributions were identified in the alveolar epithelium of the fibrotic models, suggesting abnormal remodeling.