Presentation Information
[20a-A305-8]Application of bio-speckle on micro-bioassay with Zooplankton and phytoplankton
〇(D)Devi Arti Devi1, Hirofumi Kadono1, Uma Maheswari Rajagopalan2 (1.Graduate School of Science and Engineeing Saitama University, 255 shimookubo, Sakura ward, Saitama, 338-0825, Japan, 2.Department of mechanical Eng. Faculty of Engineering, Shibaura Institute of Technology, Japan)
Keywords:
Biospeckle,Bioassay,water toxicity
Recently, a micro-bioassay has gained a lot of attention for assessing water pollution toxicity by observing the reaction of microorganisms without identifying chemicals. However, in this technique, a microscope observation is required to determine vital characteristics of plankton such as alive/dead status and swimming capability. As organisms become smaller, observation becomes harder due to the narrow focal depth of the imaging system. Here, we proposed a novel technique for micro bioassay that utilizes a biospeckle in the diffraction field generated from the plankton to overcome the difficulties in the observation of smaller micro-organisms. We employed P. chilomonas, a microorganism about 20-30µm in size. The advantage of the method is that owing to the non-imaging system, an enormous number of plankton can be processed, and a statistically significant estimation of the swimming ability of plankton under environmental pollution can be derived immediately.