[20p-A601-1~16] 3.1 Basic optics and frontier of optics (merged with formerly 3.2 Equipment optics and materials)

3:30 PM - 3:45 PM JST(6:30 AM - 6:45 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[20p-A601-10] High-speed volumetric fluorescence imaging using a visible Bessel beam with reduced sidelobes

〇(M2)Daisuke Kume^1,2, Yuichi Kozawa¹, Yuuki Uesugi¹, Shunichi Sato¹ (1.IMRAM, Tohoku Univ., 2.Grad. Sch. of Eng., Tohoku Univ.)

Keywords:

confocal laser scanning microscope,Bessel beam,computer-generated hologram

We have been developing a fast three-dimensional imaging method, which does not require the movement of the observation plane by using excitation with a Bessel beam and controlling fluorescence signals based on a computer-generated hologram. This method was successfully applied to two-photon excitation microscopy. However, applying this method to one-photon excitation microscopy suffers from the degradation of image contrast and undesired artifacts in acquired images. In this presentation, we will report a new approach to overcome these problems by applying additional control to the Bessel beam.