Presentation Information
[20p-P07-42]Extended Gate Field Effect Transistor Biosensor for fast detection of Pseudomonas
〇(M1)You Lei1, Shih Jie-Yun1, Lin Tung-Yi2, Lu Hsin-Chun1 (1.Chang Gung Univ., 2.Chang Gung Memorial Hospital)
Keywords:
extended gate,Pseudomonas aeruginosa,biosensor
Extended gate field-effect transistor (EGFET) biosensors were fabricated and used for the specific detection of Pseudomonas aeruginosa (P. aeruginosa) exotoxin A (PEA). The extended gate (EG) detection area was fabricated by depositing a gold layer by DC magnetron sputtering on the ITO conductive glass. The gold surface was then enclosed with epoxy resin and PDMS and functionalized with lipoic acid, EDC/NHS solution, and PEA capture antibodies. The resulting EGs that contained PEA capture antibodies were connected to CD4007 field effect transistors (FETs) to finish the assembly of extended gate field-effect transistor (EGFET) biosensors for PEA detection. After specific binding reactions with PEAs, the PEA-bound EGFET biosensors were connected to Keithley 2612B to measure the characteristic curves of the resulting the PEA-bound EGFETs and to obtain the quantitative relationship between the deviation of the turn-on voltage and PEA concentration. The experiment results showed that the detection range of the PEA EGFET biosensors was between 97.6 ng/mL and 100000 ng/mL and the linear correlation coefficient R2 was 0.99 when the EGs were bound with PEA capturing antibodies with a saturation concentration of 57000 ng/mL. The interference test results showed that the existence of Protein A and BSA did not affect the detection range and the linear correlation between the deviation of the turn-on voltage and PEA concentration. The overall detection time of PEA by the developed PEA EGFET biosensors was less than 40 minutes, and the detection limit of the PEA EGFET biosensors was 97.6 ng/mL, which is much smaller than the pathogenic concentration corresponding to P. aeruginosa infection of 332 μg/mL. These results showed that the EG FET biosensors developed in this research have the potential for rapid clinical diagnosis of P. aeruginosa infection.