Presentation Information
[20p-P10-6]Development of Gene-Based Rapid Test Device for Bloodstream Infection
〇Saaya Shimizu1, Yoshimitsu Yanagawa1, Ryo Imai1, Tomoyuki Sakai1 (1.Hitachi, Ltd.)
Keywords:
microfluidics,bacterial test,gene-based test
To achieve bacterial test for bloodstream infection within one hour, we are developing gene-based testing system using microfluidic device. Here, we report a device that integrates three steps of the testing flow: DNA amplification, DNA single-stranding, and DNA detection. We selected a method for each step of the flow and developed an integrated microfluidic device. We tested detection of 100 copies bacterial DNA assuming blood sample from patients with bloodstream infection. As a result, we achieved detection of the targeted bacterial DNA in about 14 minutes.