[17a-B4-1~9] 6.6 Probe Microscopy

11:00 AM - 11:15 AM JST(2:00 AM - 2:15 AM UTC)

[17a-B4-9] Mass Spectrometry Imaging of Human Heart Disease Tissue by Tapping-Mode Scanning Probe Electrospray Ionization

〇Yoichi Otsuka^1,2, Shuichi Shimma³, Hidetaka Kioka⁴, Tomohito Ohtani⁴, Yasushi Sakata⁴ (1.Sci., Osaka Univ., 2.FRC, Osaka Univ., 3.Eng., Osaka Univ., 4.Med., Osaka Univ.)

Keywords:

Biological tissue,Heart disease,mass spectrometry imaging

Mass spectrometry imaging of human dilated cardiomyopathy, an intractable disease, was performed using tapping-mode scanning probe electrospray ionization. Lipids localized in fibrotic regions and widely distributed in the myocardium were visualized. Furthermore, we found that the ion signal intensity of a specific group of lipids related to mitochondrial function was markedly reduced in normal perifibrotic cardiomyocytes.