[17p-C32-1~13] 12.6 Nanobiotechnology

4:30 PM - 4:45 PM JST(7:30 AM - 7:45 AM UTC)

[17p-C32-13] Fluorescence microscopy of single extracellular vesicles released from cultured breast cancer cells

〇(M2)Makoto Tokami¹, Yasunori Nawa¹, Hajime Shigeto², Shohei Yamamura², Keiko Tawa¹ (1.Kwansei Gakuin Univ., 2.HMRI, AIST)

Keywords:

Plasmon,Single Exosome,Fluorescence microscopy

Exosomes (Evs) released from cells can be used for disease diagnosis and prediction. In our laboratory, we have established an immunoassay to capture single Evs isolated from serum using a plasmonic chip with fluorescence enhancement effect, and quantitatively evaluated them as bright spots under a fluorescence microscope. In this study, breast cancer cells MCF-7 seeded were incubated in dishes for several days and Evs released from cells were quantitatively evaluated by immunoassay on the plasmonic chip.