[17p-C32-1~13] 12.6 Nanobiotechnology

3:00 PM - 3:15 PM JST(6:00 AM - 6:15 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[17p-C32-8] Evaluation of viable bacteria counts based on the electrochemical response of tetrazolium salts

〇Akira Tokonami¹, Ikeda Hikaru¹, Fujimura Hayato¹, Sadanaga Yasuhiro¹, Shiigi Hiroshi¹ (1.Osaka Metropolitan Univ.)

Keywords:

tetrazolium salts,Electrochemical Measurement,viable cell count

MTT, a type of tetrazolium salt, is membrane-permeable and reacts with electron transporters in living cells, where it is reduced to insoluble formazan, which is deposited intracellularly. In this study, we developed a method to evaluate viable cell counts by focusing on the electrochemical response of MTT remaining in bacterial suspensions, which shows a characteristic redox-based current response. The number of viable cells can be evaluated by focusing on the reduction current and quantifying the amount of MTT remaining in the suspension without being taken up by viable cells.