[18a-C43-1~9] 1.1 Interdisciplinary and General Physics

10:00 AM - 10:15 AM JST(1:00 AM - 1:15 AM UTC)

[18a-C43-5] Characterization of biospeckle for micro-bioassay using mycelium

〇(M1)Shuntaro Nakasugi¹, Devi Arti¹, Hirofumi Kadono¹ (1.Saitama univ.)

Keywords:

Biospeckle,Bioassay,Mycelium

With more than 200 million chemical substances currently registered, methods to identify individual chemical substances and evaluate their toxicity individually are impractical due to the enormous time and cost involved. Therefore, a method called bioassay, which evaluates toxicity based on the response of biological materials exposed to contaminants, is attracting attention. Among these methods, those that use microorganisms as biological materials are called micro-bioassays. In this method, it is necessary to determine whether the microorganisms are alive or dead by microscopic observation, but there are some problems: for example, the higher the magnification of the microscope, the shallower the depth of focus becomes, and the more the microorganisms move out of the observable range.
We have been validating the effectiveness of the biospeckle microbioassay method using plankton by using an optical method that does not use an imaging system, which we originally proposed[1]. In this study, we tested the validity of the method for the assessment of zinc toxicity by culturing the hygrophilic fungus cladosporium in a zinc environment, a type of heavy metal.