[19p-C32-1~12] 3.3 Biomedical optics

1:00 PM - 1:15 PM JST(4:00 AM - 4:15 AM UTC)

[19p-C32-1] Optimization of control beams for super-resolution stimulated Raman scattering microscopy with photoswitchable Raman probes

〇Hikaru Akaboshi¹, Takaha Mizuguchi², Spencer J. Spratt², Kazuhiro Kuruma², Hiroyoshi Fujioka³, Minoru Kawatani³, Mako Kamiya³, Yasuyuki Ozeki^1,2 (1.EEIS, UTokyo, 2.RCAST, UTokyo, 3.Tokyo Tech)

Keywords:

Raman,microscopy,super-resolution

To understand the detailed mechanisms of biological phenomena, it is crucial to image multiple targets within cells at high spatial resolution. Previously, we proposed a super-resolution imaging method utilizing a photoswitchable Raman probe and achieved super-resolution imaging with a spatial resolution of approximately 150 nm. In this report, we successfully optimized the irradiation conditions of the control light, reducing the control light power to below 1 µW.