[19p-C32-1~12] 3.3 Biomedical optics

1:45 PM - 2:00 PM JST(4:45 AM - 5:00 AM UTC)

[19p-C32-4] Raman spectroscopic imaging of rapidly frozen biological samples

Kenta Mizushima^1,2, Kosuke Tsuji^1,2, Shoko Tamura⁴, Masahito Yamanaka¹, Kentaro Mochizuki⁴, Menglu Li¹, Syusuke Egoshi⁵, Kosuke Dodo⁵, Yoshinori Harada⁴, Nicholas Smith⁶, Mikiko Sodeoka⁵, Hideo Tanaka⁴, 〇Katsumasa Fujita^1,2,3 (1.Osaka Univ., 2.AIST, 3.OU-OTRI, 4.KyotoPrefUnivMed., 5.RIKEN, 6.OU-IFReC)

Keywords:

Raman microscopy,Cryo-optical Microscopy

Raman spectroscopic imaging analyzes molecular components within a sample and provides their spatial distribution. However, due to the weak nature of Raman scattering, detecting low-concentration molecules and accurately capturing the morphology of moving samples have been challenging. In this study, we developed a cryo-Raman microscope that rapidly freezes cells and maintains a stable low-temperature environment, enabling long-term observation. This system improves the signal-to-noise ratio and resolution, and it reveals Raman scattering peaks that are not observable at room temperature. This technology is expected to enhance the utility of Raman spectroscopic imaging in life sciences and basic medical research.