[20a-A25-1~10] 3.3 Biomedical optics

9:30 AM - 9:45 AM JST(12:30 AM - 12:45 AM UTC)English Presentation

[20a-A25-3] Evaluation of water toxicity using a fast and reliable novel biospeckle micro bioassay technique

〇(DC)Devi Arti Devi¹, Hirofumi Kadono¹, Uma Maheswari Rajagopalan² (1.Graduate School of Science and Engineeing Saitama University, 255 shimookubo, Sakura ward, Saitama, 338-0825, Japan, 2.Department of mechanical Eng. Faculty of Engineering, Shibaura Institute of Technology, Japan)

Keywords:

Biospeckle,water pollution,Bioassay,microorganisms,water toxicity,Cd

Recently, micro bioassay has been getting a lot of attention, where the toxicity of water pollution is assessed based on the reaction of microorganisms without identifying individual chemical substances. In this technique, a microscope observation is necessary to get their critical features such as alive/dead status, and swimming ability. As the size of the microorganism becomes smaller, observation becomes more difficult due to the narrower focal depth of the imaging system. In our study, to overcome these difficulties, we proposed a novel technique based on biospeckle for micro bioassay, where the change in biospeckle is focused on monitoring the motor ability of microorganisms in response to the toxicity of the environment. Our previous studies have successfully validated this technique using microorganisms exposed to various concentrations of environmental pollutants such as iron (Fe), zinc (Zn), and trichloroacetic acid. In this study, we focused on cadmium (Cd), another emerging water pollution, often resulting from industrial discharge and agricultural runoff, contaminates water bodies, posing serious health risks and harming aquatic ecosystems. Therefore, it is significant to know the response of microorganisms for Cd as well.