[20a-P03-1~29] 12.6 Nanobiotechnology

[20a-P03-11] Viability assessment of bacterial cells based on light scattering properties

〇Akira Tokonami¹, Kawanaka Miya², Ikeda Hikaru¹, Sadanaga Yasuhiro^1,2, Shiigi Hiroshi^1,2 (1.Osaka Metropolitan Univ., 2.Osaka Prefecture Univ.)

Keywords:

cell activity,Scattered light measurement,Dark-field microscopy

MTT, a type of tetrazolium salt, is membrane permeable and reacts with electron transporters in living cells, where it is reduced to insoluble formazan, which is deposited intracellularly. Under dark-field microscopy, more active cells produced more formazan, and the formation of red crystal particles was more pronounced. We focused on the light scattering properties of these crystalline particles and succeeded in quantifying the activity of single bacterial cells. In this presentation, we discuss the effects of various bactericidal treatments.