[20a-P03-1~29] 12.6 Nanobiotechnology

[20a-P03-13] Spatial-temporal measurement of cardiomyocytes membrane fluidity

〇Yuga Yamada¹, Hiroshi Yoshikawa¹, Yoshihiro Ujihara², Takahisa Matsuzaki¹ (1.Osaka Univ., 2.Nagoya Institute of Technology)

Keywords:

membrane fluidity,cardiomyocytes,Fluorescence Imaging

Cardiomyocytes are regularly beaten by cyclical interaction between molecular motors, propagating “via” the cell membrane to neighboring cells. However, it has not been clarified how the physical properties of the cellular membrane control the beating of the cardiomyocyte tissues. In this study, we quantitatively visualized the membrane fluidity of normal cardiomyocytes during the maturation process. To evaluate membrane fluidity, we applied 2-dimethylamino-6-lauroylnaphthalene (laurdan) fluorophores for the cells that can evaluate membrane fluidity based on the fluorescence peak shifts. We found that membrane fluidity reduced stepwise according to the maturation of cardiomyocytes. Interestingly, we also identified the presence of small fibers inside the cells as the origin of the reduced membrane fluidity. The fibers are called T-tubules, we suggested that the presence of reduced fluidic nanofibers plays key roles in regular heart beating.