[20a-P03-1~29] 12.6 Nanobiotechnology

[20a-P03-14] Measurement of Mechanical Distribution of organs by Atomic Force Microscopy

〇Momoka Horikiri¹, Kanako Iwamori², Hiroshi Yoshikawa¹, Fukada So-ichiro², Matsuzaki Takahisa¹ (1.Grad. Sch. Eng., Osaka Univ., 2.Grad. Sch. Pharm., Osaka Univ.)

Keywords:

muscle,measurement

Organs in vivo have inherent mechanics (Young’s modulus E kPa) that are controlled in an appropriate balance, and the role of mechanics during regeneration has attracted much attention. To unveil the roles of mechanics in skeletal muscle regeneration, we have established a methodology for simultaneous measurement of mechanics and its biological origins using atomic force microscopy and confocal microscopy. We revealed that the skeletal tissues decrease the mechanics from day 0 to 5 and gradually increase the mechanics toward day 14. Simultaneously, we obtained the biological origins of the stiffening is an extracellular matrix supporting the regeneration of muscle myofibers. The results suggest that mechanics can be a physical indicator of the degree of skeletal muscle regeneration. In the poster presentation, we will discuss the mechanism of stiffening and mechanical distribution during skeletal muscle and compare the model of skeletal muscle regeneration failure.