[20a-P04-1~10] 12.7 Biomedical Engineering and Biochips

[20a-P04-3] Generation of pacemakers in cardiomyocyte aggregates by mechanical stimulation using a Real-Time Feedback control

〇Arisa Mizutani¹, Kyotaro Kanazashi¹, Ayu Sasaki¹, Yuuta Moriyama^1,2, Toshiyuki Mitsui¹ (1.Aogaku Univ., 2.PRESTO, JST)

Keywords:

cardiac muscle cells,real-time feedback,biophysics

We constructed a feedback system to observe the intrinsic beating activity of cardiac cells in real-time and attempted to generate pacemaker-like stimuli in an aggregation of these cells by phase-controlling the timing of stimulation. In this experiment, we prepared cell aggregates from 5-day-old chicken embryos and formed them into a linear shape. The phase differences between the stimulus and the intrinsic beat were crucial for controlling the interbeat interval and shifting the pacemaker cells.