Presentation Information
[22a-P03-9]Localized lipid membrane fusion controlled by a chemical pen system
〇Huan Luo1, Nahoko Kasai1, Hizuru Nakajima1, Shungo Kato1, Katsumi Uchiyama1, Sifeng Mao1 (1.Tokyo Metro. Univ.)
Keywords:
Chemical pen,Membrane fusion,Localized control
Membrane fusion is a lipid-centric event and one of the most fundamental processes in biology for intracellular transport, viral infection, hormone secretion and neurotransmitter release. A proper understanding of membrane fusion would help precisely regulate cellular processes, and pave the way for breakthroughs in targeted drug delivery. In this work, a self-spreading supported lipid bilayer (SLB) was prepared to mimic the typical biological membranes, small unilamellar lipid vesicles (SUVs) were prepared to mimic extracellular vesicles, a chemical pen system with two injection apertures and one aspiration aperture was developed for localized membrane fusion control. The two injected flows are hydrodynamically confined and formed a mixing region to create a localized experimental condition. Thus, the membrane fusion can be precisely controlled and monitored at this designed local region. This work furtherly confirmed the acidic condition triggered lipid membrane fusion and provided important insights about fusion mechanisms.