Presentation Information
[23a-12C-6]Fast and reliable micro bioassay techniques for organic solvent based on biospeckle for swift water assessment using plankton
〇(D)Devi Arti Devi1, Hirofumi Kadono1, Uma Maheswari Rajagopalan2 (1.Graduate School of Science and Engineeing Saitama University, 255 shimookubo, Sakura ward, Saitama, 338-0825, Japan, 2.Department of mechanical Eng. Faculty of Engineering, Shibaura Institute of Technology, Japan)
Keywords:
Biospeckle,Bioassay,organic water solvent
Recently, micro-bioassay has been getting attention where the toxicity of water pollution is assessed based on the reaction of micro-organisms such as plankton without identifying chemicals. In this technique, a microscope observation is necessary to get their critical features such as alive/dead, and swimming ability. As the size of the micro-organism becomes smaller, observation becomes more difficult due to the narrower focal depth of the imaging system. In our study, to overcome the difficulties, we proposed a novel technique of Biospeckle micro bioassay, where a change of biospeckle is focused on monitoring the motor ability of plankton in response to the toxicity of the environment. Our previous study successfully validated its efficacy using plankton exposed to varying concentrations of the environmental heavy metal pollution Zn and Fe [1]. In this study, we utilized Euglena gracilis (E.gracilis) and Paramecium caudatum (P.caudatum), which has the motor ability, and investigated the effects of various concentrations of the organic solvent trichloroacetic acid (CCl3COOH) known as one of the environmental pollutants.