Presentation Information
[23a-12C-7]Biospeckle optical coherence tomography (bOCT) in the early detection of size-dependent impact of Titanium dioxide (TiO2) nanoparticles on the internal activity of Lentil (Lens culinaris) seeds
〇(D)Lavista Tyagi1, Hirofumi Kadono1, Uma Maheswari Rajagopalan2 (1.Graduate School of Science and Engineering Saitama University, 255 Shimo-Okubo, Sakura ward, Saitama 330-0825, Japan, 2.Department of Mechanical Eng. Faculty of Engineering, Shibaura Institute of Technology, Japan)
Keywords:
Biospeckle Optical Coherence Tomography (bOCT),Nanoparticles,Titanium dioxide (TiO2)
The utilization of nanoparticles (NPs) has been on the rise considerably in the medical, industrial, as well as agricultural sectors. The techniques to monitor the response of plants to NPs are crucial since the effects of nanomaterials on seed germination and plant growth are uncertain. Additionally, recognizing that NPs can penetrate seeds and influence cellular processes underscores the increased importance of comprehending their size-dependent effects. Here, we proposed the Biospeckle Optical Coherence Tomography (bOCT), developed in our lab to observe the size-dependent effect of Titanium dioxide (TiO2) NPs on the internal activity of lentil (Lens culinaris) seeds considering TiO2 is one of the most engineered nanomaterials and possesses significant potential for accumulation in the food chain due to its extensive applications. bOCT is a non-contact, non-destructive, in vivo monitoring technique to visualize the change of internal activity of a biological object. Our earlier study effectively confirmed its ability to assess the size-dependent effects of ZnO NPs, indicating a substantial negative impact of ZnO NPs with smaller sized <50nm compared to those <5µm.