Presentation Information

[23a-12F-8]Mass Spectrometry Imaging of Mouse Retina by Tapping-mode Scanning Probe Electrospray Ionization

〇(D)Mengze Sun1, Yoichi Otsuka1, Maki Okada1, Katsuyuki Nagata2, Heido Shindou2, Michisato Toyoda1 (1.Graduate school of science, Osaka Univ, 2.National Center for Global Health and Medicine)


Mass spectrometry imaging,Scanning probe,Lipid

Retinas play an essential role in visual function, such as in humans and mammals. Inside the retina, photoreceptor cells receive light signals and convert them into neural signals. Photoreceptor cells require a specialized lipid metabolism to maintain their structure and function. Saturated phosphatidylcholine (PC), which consists of two saturated acyl chains and a polar choline head group, is primarily present in the retina. The previous reports have shown that mice carrying a frameshift mutation and deficiency in the gene of lysophospholipid acyltransferase 8 (LPLAT8), which produces saturated PC with lysophosphatidylcholine (LPC) and saturated fatty acyl-CoA, showed spontaneous retinal degeneration. Because the structure of the retina is complex, understanding changes in lipid distribution at the cellular scale is necessary to clarify and elucidate the status of degeneration. Mass spectrometry imaging (MSI) is a powerful method to visualize the multicomponent distribution in a tissue section. We have developed tapping-mode scanning probe electrospray ionization (t-SPESI), an ambient extraction and ionization technique, to visualize lipid distributions inside tissues without special pretreatment. In this study, we intend to compare different ion images of different lipids to clarify the relationship between retinal degeneration and molecular changes.