Presentation Information
[23a-1BN-1]Hybrid Gramicidin-ATP Synthase Biotransducer for Proton Logic gate
〇(D)Yukun Chen1, Gabor Mehes1, Bingfu Liu1, Mingyin Cui1, Noriyo Mitome2, Takeo Miyake1 (1.Waseda Univ., 2.Tokoha Univ.)
Keywords:
Biotransducer,ATP synthase,Gramicidin A
Introduction and Motivation:
In the realm of microfabricated bioelectrical devices (biodevices), bridging the gap between biological and electrical systems is a frontier yet to be fully explored. In our previous research, we demonstrated protonic biotransducer, modulated enzyme cascade reactions[1], mitochondrial organelle[2] and cell function[3], we have advanced the technology to include bidirectional ion transport. This study presents a novel biotransducer that incorporates ATP synthase and the proton channel gramicidin A (gA), integrated into supported lipid bilayers (SLBs) on a microelectrode coated with sulfonated polyaniline (SPA). By applying voltage to the SPA and introducing chemical modulators, we successfully regulated proton transport in gA and ATP synthase, creating a XOR hybrid enzyme logic system.
Results and Discussions:
Our findings include the development of a capacitive bioelectrical system, validated by electrochemical impedance spectroscopy (EIS), and a functional XOR logic gate activated by chemical and electrical inputs, showcasing the dynamic control of ion currents across SLBs. This hybrid system not only enhances our understanding of ion transport mechanisms but also signals a significant step towards intricate biochemical computing systems. The integration of this system with other biological components could lead to the development of advanced biodevices for medical and biotechnological applications. These achievements underscore the potential of gA-ATP synthase hybrid systems in bioiontronics, setting the stage for future innovations in the field.Reference[1] Y. Chen, M. Cui, C. Lin, B. Liu, N. Mitome, T. Miyake, Adv Materials Technologies 2022, 7, 2100729.[2] Z. Zhang, H. Kashiwagi, S. Kimura, S. Kong, Y. Ohta, T. Miyake, Sci Rep 2018, 8, 10423.[3] M. Cui, M. Takahashi, Y. Chen, B. Liu, Y. Ohta, T. Miyake, Bioelectrochemistry 2022, 147, 108202.
In the realm of microfabricated bioelectrical devices (biodevices), bridging the gap between biological and electrical systems is a frontier yet to be fully explored. In our previous research, we demonstrated protonic biotransducer, modulated enzyme cascade reactions[1], mitochondrial organelle[2] and cell function[3], we have advanced the technology to include bidirectional ion transport. This study presents a novel biotransducer that incorporates ATP synthase and the proton channel gramicidin A (gA), integrated into supported lipid bilayers (SLBs) on a microelectrode coated with sulfonated polyaniline (SPA). By applying voltage to the SPA and introducing chemical modulators, we successfully regulated proton transport in gA and ATP synthase, creating a XOR hybrid enzyme logic system.
Results and Discussions:
Our findings include the development of a capacitive bioelectrical system, validated by electrochemical impedance spectroscopy (EIS), and a functional XOR logic gate activated by chemical and electrical inputs, showcasing the dynamic control of ion currents across SLBs. This hybrid system not only enhances our understanding of ion transport mechanisms but also signals a significant step towards intricate biochemical computing systems. The integration of this system with other biological components could lead to the development of advanced biodevices for medical and biotechnological applications. These achievements underscore the potential of gA-ATP synthase hybrid systems in bioiontronics, setting the stage for future innovations in the field.Reference[1] Y. Chen, M. Cui, C. Lin, B. Liu, N. Mitome, T. Miyake, Adv Materials Technologies 2022, 7, 2100729.[2] Z. Zhang, H. Kashiwagi, S. Kimura, S. Kong, Y. Ohta, T. Miyake, Sci Rep 2018, 8, 10423.[3] M. Cui, M. Takahashi, Y. Chen, B. Liu, Y. Ohta, T. Miyake, Bioelectrochemistry 2022, 147, 108202.