[23p-12C-1~13] 3.3 Biomedical optics (formerly 3.4)

3:30 PM - 3:45 PM JST(6:30 AM - 6:45 AM UTC)English Presentation

[23p-12C-8] Label-Free Organelle Imaging by Hyperspectral Stimulated Raman Scattering and Deep Learning

〇(M2)Yang Ma¹, Takaha Mizuguchi¹, Spencer Spratt¹, Yasuyuki Ozeki¹ (1.UTokyo)

Keywords:

Raman microscopy,Deep learning,Fluorescence imaging

This study develops a deep learning model for label-free imaging of cell organelles using hyperspectral Stimulated Raman Scattering (SRS) images. It combines high spectral resolution and a large number of spectral points for accurate organelle prediction. The model, evaluated using Mean Squared Error, shows promising results in cellular dynamics understanding with minimal perturbation.