Presentation Information

3:45 PM - 4:00 PM JST(6:45 AM - 7:00 AM UTC)

[23p-12C-9]Three-Dimensional Imaging of Biological Samples by Needle Scanning Fluorescence Microscopy Using a Visible Bessel Beam

Daisuke Kume^1,2, 〇Yuichi Kozawa¹, Yuuki Uesugi¹, Shunichi Sato¹ (1.IMRAM, Tohoku Univ., 2.Grad. Sch. of Eng., Tohoku Univ.)

Keywords:

Laser scanning microscopy,Spatial light modulator,Bessel beam

We are currently developing laser scanning microscopy utilizing a Bessel beam with an extended focal depth, with the wavefront modulation for fluorescence signals, achieving the three-dimensional image reconstruction from a two-dimensional scanning of the excitation spot. Here, we demonstrate three-dimensional visualization of biological samples using this principle based on an intensity-modulated, visible Bessel beam. The detailed imaging performance of the present method is also evaluated.

Back to Session information