[23p-1BM-1~16] 12.7 Biomedical Engineering and Biochips

3:15 PM - 3:30 PM JST(6:15 AM - 6:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[23p-1BM-7] Fabrication of enzyme-based image sensor for measuring sucrose in plants

〇Yusuke Matsushita¹, Hideo Doi¹, Kotaro Takayama¹, Yong-Joon Choi¹, Kazuhiro Takahashi¹, Kazuaki Sawada¹, Toshihiko Noda¹ (1.Toyohashi Univ. of Tech.)

Keywords:

Redox potential detection array sensor,Enzyme,Sucrose

Using a cross-linking polymer, redox-enzymes for sucrose detection were immobilized on a 30 µm-pitch potentiometric redox array sensor on which a gold and titanium layer film was deposited, and the sucrose response was investigated. Sucrose concentration dependence was measured on the enzyme-immobilized membrane and the sensitivity was 35.5 mV/dec in the range of 10^-4 to 10^-2 M. When a stem slice was placed on the sensor surface, the output images as a high concentration of sucrose in the stem outer frame area was obtained, and successfully detected the sucrose distribution in the plant body.