Presentation Information
[24a-1BM-1]Development of Molecularly Imprinted Polymer Nanoparticle with High Affinity
and Its Application to FET Biosensors
〇(D)Youyuan Man1, Nishitani Shoichi2, Sakata Toshiya1 (1.Tokyo Univ., 2.California Univ.)
Keywords:
Molecularly Imprinted Polymer,FET,Biosensor
Glycated albumin (GA) has recently been extensively studied as a biomarker, aiming for a 2-week blood sugar assessment for diabetes patients, which addresses issues before they are evident during the 3-month span from glycated hemoglobin (HbA1c). While the traditional immunochemical methods used for measuring albumin levels faced challenges like antibodies which are expensive to produce and purify, the introduction of biosensors, especially those deploying field-effect methods, showed promise. However, these techniques brought their own challenges, notably the need for specific surface materials. In search of a cost-effective solution, molecularly imprinted polymers (MIPs) emerged, however, often results in heterogeneous binding sites with possibly reduced target molecule affinity using conventional synthesis methods. To address this issue, an approach using solid-phase to synthesize MIP nanoparticles (nano-MIPs) has been recently developed. Human serum albumin (HSA) was used as a template in this study due to its cost-effectiveness to conduct more laboratory studies. Moreover, as a precursor to GA, HSA is capable of providing a promising foundation for templating GA in the future.