Presentation Information
[10p-N203-10]Label-Free Detection of Glucose and Lactate Using Terahertz Chemical Microscope with Aptamer-Functionalized Sensing Plates
〇Yuta Mitsuda1, Ryotaro Kubo1, Hikaru Nakagawa1, Toshihiko Kiwa1, Jin Wang1 (1.Graduate school of Okayama Univ.)
Keywords:
Terahertz,Aptamer,Biosensing
Glucose and lactate are essential biomarkers for monitoring diabetes and lactic acidosis. Conventional detection methods often require labeling or enzymatic reactions, which are time-consuming and may lack sensitivity. In this study, we demonstrate a novel, label-free approach for detecting glucose and lactate using a Terahertz Chemical Microscope (TCM) integrated with aptamer-functionalized Silicon-on-Sapphire sensing plates. Upon exposure to glucose or lactate, changes in terahertz amplitude were measured using a photoconductive antenna and femtosecond laser system. The amplitude showed a strong correlation with analyte concentration. Glucose detection exhibited a monotonic increase in signal, while lactate showed responses over a wide range (0.1–100 mM). This approach enables real-time, reproducible, and sensitive detection without the need for labeling or enzymes. It holds promise for non-invasive monitoring of clinically important biomarkers such as glucose and lactate.