[10p-N305-1~12] 12.7 Biomedical Engineering and Biochips

3:15 PM - 3:30 PM JST(6:15 AM - 6:30 AM UTC)Presentation by Applicant for JSAP Young Scientists Presentation Award

[10p-N305-7] The use of an open-top microfluidic chip for rapid freezing of biological cells

〇Yoshino Tanabe¹, Kosuke Tsuji¹, Takumi Kunimoto¹, Masahito Yamanaka¹, Katsumasa Fujita^1,2 (1.UOsaka, 2.UOsaka OTRI)

Keywords:

microfluidic chip,cryo-optical microscopy,rapid freezing

We have developed the "time-deterministic cryo-optical microscope," a technology that enables rapid freezing of live cells with millisecond-level time precision during live imaging with optical microscopes. It allows for the visualization of cellular morphology, biomolecular distribution, chemical states, and ion concentration in a near-native state, making spatiotemporal analysis of rapid frozen cells possible. Achieving optimal freezing conditions requires precise control of buffer volume within the sample. In this study, we proposed and evaluated a liquid volume control technique utilizing an open-top microfluidic chip.