Presentation Information
[7a-N306-1]Sensitive detection of Biotin-Avidin interaction using a plasmonic multi-array with capture molecule by photochemical reaction
〇Moe Taniguchi1, Kyohei Asano1, Yasunori Nawa1, Mutsuo Tanaka2, Keiko Tawa1 (1.Kwansei Gakuin Univ., 2.Saitama Inst. of Tech.)
Keywords:
plasmon,photochemical reaction,biosensor
The plasmonic chip is a substrate with a wavelength-sized periodic structure covered with thin metal layers. In our laboratory, the electric field enhanced with the Bull’s eye-type plasmonic chip was applied to not only the fluorescence enhancement but also the promotion of photochemical reaction. The chip modified to photo-responsive molecule (4-((2-folmyl-3-methylphenyl)thio) benzoic acid) (B-O-MBA) was irradiated with visible light, and intermediates of B-O-MBA were formed in the exposed area. On the structure of plasmonic chip, this photochemical reaction is promoted and maleimide compounds are densely bound. We aim to construct multi-array type sensitive biosensors by promotion of photochemical reactions and fluorescence enhancement with plasmonic chips. In this study, biotin-maleimide (Biotin-M) was bonded to the B-O-MBA to form a capture interface, and the biotin-avidin interaction was quantitatively evaluated by fluorescence image of Cy5-labeled streptavidin (Cy5-SA).