Presentation Information
[7a-N306-3]Preparation of 2D array plasmonic chip for high-sensitivity fluorescence measurement of biological sample
Atsushi Shimizu1, 〇Yasunori Nawa1, Hajime Shigeto2, Shohei Yamamura2, Keiko Tawa1 (1.Kwansei Gakuin Univ., 2.HMRI, AIST)
Keywords:
Inverted epifluorescence microscope,Enhanced fluorescence,Plasmonic chip
Fluorescence microscopy is a powerful tool for visualizing biological functions. We have developed a plasmonic chip capable of utilizing grating-coupled surface plasmon resonance (GC-SPR) under a microscope, enabling highly sensitive fluorescence imaging of tumor markers, lifestyle-related disease markers, and cells. In this study, we fabricated a deep-hole array plasmonic chip with optical transparency, suitable for use with inverted microscopes. Using this chip, fluorescent microspheres with a diameter of 0.2 µm were clearly visualized with a high signal-to-noise ratio, and the fluorescence intensity was approximately 64 times higher than that of the conventional structure.