[7p-N322-1~14] CS.2 Code-sharing session of 3.2&4.4

4:15 PM - 4:45 PM JST(7:15 AM - 7:45 AM UTC)English Presentation

[7p-N322-10] [JSAP-Optica Joint Symposia Invited Talk] Observation of Tobacco-cultured Cells by using Three-dimensional Fluorescence Imaging Based on Transport of Intensity Equation and Prospects for Clarifying the Effects of Terahertz Waves on Cells

〇Shiori Matsuda¹, Safumi Suzuki¹, Wataru Watanabe², Osamu Matoba^3,4 (1.Science Tokyo, 2.Ritsumeikan Univ., 3.Grad. Sch. of System Informatics, Kobe Univ., 4.OaSIS, Kobe Univ.)

Keywords:

fluorescence imaging,biological imaging,terahertz

Fluorescence imaging is a powerful technique for visualizing internal structures in living organisms. Recently, three-dimensional (3D) imaging based on the transport of intensity equation (TIE) has shown promise. However, image quality suffers from tissue scattering. We developed a method combining TIE-based fluorescence imaging with iterative phase retrieval to reduce scattering effects, enabling clearer 3D visualization. This technique is expected to serve as a useful tool for investigating cellular responses to terahertz (THz) illumination. In this study, we present 3D fluorescence imaging results of plant cells and discuss the potential application of this technique to the investigation of cellular responses under THz irradiation.